منابع مشابه
COP-coated vesicles
Approximately one third of a cell's proteins are destined to function outside the cell's boundaries or while embedded within cellular membranes. Ensuring these proteins reach their diverse final destinations with temporal and spatial accuracy is essential for cellular physiology. In eukaryotes, a set of interconnected organelles form the secretory pathway, which encompasses the terrain that the...
متن کاملTwo Human ARFGAPs Associated with COP-I-Coated Vesicles
ADP-ribosylation factors (ARFs) are critical regulators of vesicular trafficking pathways and act at multiple intracellular sites. ADP-ribosylation factor-GTPase-activating proteins (ARFGAPs) are proposed to contribute to site-specific regulation. In yeast, two distinct proteins, Glo3p and Gcs1p, together provide overlapping, essential ARFGAP function required for coat protein (COP)-I-dependent...
متن کاملArabidopsis Sec21p and Sec23p homologs. Probable coat proteins of plant COP-coated vesicles.
Intracellular protein transport between the endoplasmic reticulum (ER) and the Golgi apparatus and within the Golgi apparatus is facilitated by COP (coat protein)-coated vesicles. Their existence in plant cells has not yet been demonstrated, although the GTP-binding proteins required for coat formation have been identified. We have generated antisera against glutathione-S-transferase-fusion pro...
متن کاملSorting by COP I-coated vesicles under interphase and mitotic conditions
COP I-coated vesicles were analyzed for their content of resident Golgi enzymes (N-acetylgalactosaminyltransferase; N-acetylglucosaminyltransferase I; mannosidase II; galactosyltransferase), cargo (rat serum albumin; polyimmunoglobulin receptor), and recycling proteins (-KDEL receptor; ERGIC-53/p58) using biochemical and morphological techniques. The levels of these proteins were similar when t...
متن کاملHydrolysis of bound GTP by ARF protein triggers uncoating of Golgi- derived COP-coated vesicles
The cycle of nucleotide exchange and hydrolysis by a small GTP-binding protein, ADP-ribosylation factor (ARF), helps to provide vectoriality to vesicle transport. Coat assembly is triggered when ARF binds GTP, initiating transport vesicle budding, and coat disassembly is triggered when ARF hydrolyzes GTP, allowing the uncoated vesicle to fuse.
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ژورنال
عنوان ژورنال: Current Biology
سال: 2016
ISSN: 0960-9822
DOI: 10.1016/j.cub.2015.12.017